Induction of final maturation by sperm penetration in canine oocytes
نویسندگان
چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation
OBJECTIVE The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca2+ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). METHODS MII-stage and arrested MI-stage mouse oocytes after IVM wer...
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This study was designed to evaluate the relationship between sperm factors and penetration capacity in an in vitro system with immature porcine oocytes. The sperm parameters evaluated in 145 ejaculates were volume, sperm concentration, total cells in the ejaculate, ATP content, morpho-anomalies, percentage of motile sperm cells, forward progressive motility (FPM), acrosome status (NAR), hypo-os...
متن کاملIn vitro maturation of canine oocytes: a unique conundrum
Oocyte maturation depends on multiple variables. In fact, in dogs, the requirements for oocyte development in vitro are so complex that achievement of female gamete maturation has been viewed as a difficult problem. The conundrum, thus far unanswered, of achieving dog oocyte maturation in vitro, is nowadays the major puzzle of the in vitro program in the canine species. In in vitro studies of d...
متن کاملChromatin patterns of immature canine oocytes after in vitro maturation.
In canine species, in vitro maturation (IVM) rates of oocytes collected from anoestrous ovaries are low (<20%). Several IVM media have been tested without significant improvements. A critical step in the evaluation of culture conditions is the observation of the meiotic stage reached by the oocytes. The present study was designed to investigate the chromatin patterns of in vitro matured oocytes...
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ژورنال
عنوان ژورنال: Reproduction
سال: 2001
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep.0.1210097